Objective To investigate the prevalence of qacE, qacE△1, and qacE△1-SUL1 disinfectant-resistant genes and the minimum inhibitory concentrations (MICs) of four commonly used disinfectants for carbapenem-resistant Enterobacterales (CRE), providing reference for the scientific disinfection in hospitals. Methods 93 non-duplicate CRE strains isolated from all clinical specimens in a hospital from October 2021 to March 2022 were collected. The strains were identified and tested for antimicrobial susceptibility. Polymerase chain reaction (PCR) was used to detect the presence of disinfectant-resistant genes qacE, qacE△1, and acE△1-SUL1, and the MICs of glu-taraldehyde, iodophor, "84" disinfectant (chlorine-containing disinfectant) and ethanol to CRE were determined using agar dilution method. Results 93 CRE strains were mainly Klebsiella pneumoniae (52 strains) and Ente-robacter cloacae (25 strains). The resistance rates of CRE strains to most antimicrobial drugs were up to 100%. The prevalence rates of disinfectant-resistant genes qacE, qacE△1, and acE△1-SUL1 were 72.0% (67 strains), 81.7% (76 strains) and 89.2% (83 strains), respectively. The MICs of glutaraldehyde, iodophor, "84" disinfec-tant (effective chlorine concentration) and ethanol to CRE were 500 mg/L, 625-2 500 mg/L, 250-500 mg/L and 75%, respectively. The MICs of iodophor (2 500 mg/L) to 4 CRE strains and "84" disinfectant (effective chlorine concentration 500 mg/L) to 24 CRE strains were higher than the standard strains. Conclusion The prevalence of disinfectant-resistant genes among the CRE strains isolated from this hospital is high. CRE strains are partially resistant to iodophor and "84" disinfectant. Disinfectants should be used scientifically and properly in clinical practice, so as to prevent the spread of drug-resistant bacteria in the hospital.