Objective To improve the efficiency of hospital antimicrobial management and ensure rational clinical use of antimicrobial agents with the aid of antimicrobial stewardship (AMS) empowered by digital intelligence technology in hospital. Methods Information systems such as early warning of antimicrobial indexes, closed-loop management of microbial detection information, and decision-making system of antimicrobial resistance monitoring data were applied to the traditional AMS system. Through hospital information systems (HIS) to collect data about therapeutic antimicrobial use and healthcare-associated infection (HAI) quality control indexes of hospitalized patients in a tertiary first-class public hospital in Shenzhen City before and after digital technology improvement, indexes of 2021 and 2022 were as control group (before improvement) and observation group (after improvement) respectively, improvement trend of antimicrobial management was compared. Results After upgrading and renovating the hospital information system, hospital antimicrobial management indexes improved significantly compared to before the renovation. The use rate of antimicrobial agents and the preventive use rate of antimicrobial agents in class Ⅰ incision surgery in patients in the observation group were both lower than those in the control group (27.0% vs 38.8%, 20.9% vs 23.8%, respectively, both P < 0.05). Antimicrobial use density in hospitalized patients in the observation group was lower than that in the control group ([33.27±3.03] DDDs vs [42.06±4.42] DDDs), difference was statistically significant (t=13.11, P < 0.001). The observation group had a higher qualified rate for evaluating antimicrobial medical orders compared to the control group (98.5% vs 96.8%). The pathogenic detection rate of hospitalized patients before therapeutic antimicrobial use and pathogen detection rate related to HAI diagnosis were both higher than those in the control group (87.1% vs 84.5%, 99.0% vs 95.4%, respectively), differences were both statistically significant (both P < 0.05). Conclusion Empowering the hospital's AMS system with digital technology can promote more scientific, standardized, efficient, and rational antimicrobial management in hospitals.