Staphylococcus aureus (SA) is one of the common pathogens causing healthcare-associated infection, which easily adheres to the surface of catheters or implants to form biofilm, leading to increased antimicrobial resis-tance, and posing great challenge to clinical treatment. In recent years, studies have shown that antimicrobial agents at sub-minimum inhibitory concentration(sub-MIC) can affect the biofilm formation of SA. This paper elaborates the formation process and gene regulation of SA biofilm, as well as the effect and mechanisms of different antimicrobial agents at sub-MIC on SA biofilm formation, so as to provide basis for effective control and treatment of SA biofilm-related infection.