Objective To explore the application effect of disinfection-oriented bundle management mode of environmental cleaning and disinfection quality management of intensive care unit (ICU). Methods The bundle mana-gement mode was used to intervene the cleaning and disinfection of ICU environment. January-December 2022 and January-December 2023 were classified as pre- and post-intervention periods respectively. The awareness rate of disinfection knowledge, monitoring results of environmental hygiene, detection of MDRO and healthcare-associated infection (HAI) of patients before and after intervention were compared. Results The awareness rates of disinfection knowledge and the clearance rate of fluorescent labeling on frequently-touched environmental surface increased from 68.58% and 78.45% (pre-intervention) to 88.45% and 96.44% (post-intervention), respectively, both with statistical significance (both P < 0.05). The qualification rates of bacterial culture from frequently-touched environmental surface and hands of healthcare workers (HCWs) increased from 70.63% and 87.90% (pre-intervention) to 88.36% and 94.15% (post-intervention), respectively, both with statistical significance (both P < 0.05). The detection rates of methicillin-resistant Staphylococcus aureus (MRSA) and carbapenem-resistant Acinetobacter baumannii (CRAB) from frequently-touched environmental surface were 0.49% and 1.46% (pre-intervention), as well as 0 and 0.27% (post-intervention), respectively, both with statistical significance (both P < 0.05). The incidences of HAI (4.97%) and CRAB HAI (0.77%) post-intervention were lower than pre-intervention (7.46% and 1.62%, respectively), differences were both statistically significant (both P < 0.05). Conclusion Intervention in environmental cleaning and disinfection of ICU with bundle management mode can effectively improve the effect of cleaning and disinfection in ICU, enhance cleaners' awareness on disinfection prevention and control, decrease the detection rates of MRSA and CRAB, reduce the risk of HAI, and ensure medical safety.