Objective To understand the allocation of full-time staff in healthcare-associated infection (HAI) ma-nagement and the ability in HAI information surveillance of medical institutions (MIs) in Hubei Province, and provide a basis for the quality control of HAI management in the province. Methods In July 2023, a survey was issued to the quality control centers of cities, counties and districts of the province by Hubei HAI Management Quality Control Center, and a questionnaire survey was carried out regarding the basic situation, HAI full-time staff and HAI surveillance in secondary- and above level MIs in cities and states of Hubei Province. Results A total of 476 MIs were surveyed, 328 MIs (68.91%) had adequate staff for HAI management, and 214 MIs (44.96%) carried out HAI surveillance through HAI information system. In the survey on the ability in acquiring 13 indicators of HAI quality control, MIs carrying out HAI surveillance through HAI information system was higher than those through manual surveillance. Conclusion The construction of full-time staff of HAI management in Hubei Province needs to be further strengthened, and the ability of HAI information surveillance needs to be further improved. MIs with HAI information surveillance should pay attention to the updating and iteration of HAI surveillance system, and constantly improve the monitoring function.