Objective To investigate the current status of cleaning and disinfection of endoscopic ultrasound microprobes in tertiary hospitals in Beijing. Methods 51 tertiary hospitals in Beijing were selected with convenience sampling method, and a questionnaire survey was conducted on head nurses in endoscopy centers, the investigation included pre-treatment, cleaning, disinfection, sterilization, biological testing, disinfection traceability, storage and maintenance of endoscopic ultrasound microprobes. Results Among the 51 tertiary hospitals, 33 were first-class hospitals and 18 were second-class hospitals. 76.47% (n=39) of hospitals performed bed-side pre-cleaning on ultrasound microprobes after use, bed-side pre-treatment methods for endoscopic ultrasound microprobes mainly included enzyme solution wiping (38.47%, 15/39) and ethanol wiping (30.77%, 12/39); 96.08% (n=49) of hospitals performed manual cleaning for ultrasound microprobes, with "enzyme wash-scrub-dry" as the main method (58.82%, n=30); 96.08% (n=49) of hospitals disinfected microprobes after use, with chemical disinfectant immersion as the main methods (65.31%, n=32); 43.14% (n=22) of hospitals sterilized the microprobes, and the main method of sterilization was immersion in 0.2%-0.35% peroxyacetic acid for 10-15 minutes (77.27%, 17/22); 41.18% (n=21) of hospitals regularly conducted biological monitoring on microprobes; 37.25% (n=19) of hospitals traced the cleaning and disinfection process of microprobes; 88.24% (n=45) of hospitals regularly inspected, maintained, and repaired microprobes. Conclusion The pre-treatment, cleaning, disinfection, sterilization, and biological monitoring of endoscopic ultrasound microprobes in 51 tertiary hospitals in Beijing varies significantly, and it is recommended that a relevant expert consensus or guideline be formulated as early as possible, so as to standardize the cleaning and disinfection of endoscopic ultrasound microprobes.