Objective To analyze the drug resistance and serotype of Group B Streptococcus (GBS) isolated from pediatric patients, provide reference for the prevention and treatment of GBS infection as well as vaccine development in children. Methods 163 non-repetitive GBS strains detected at Beijing Children’s Hospital of Capital Medical University from January 1, 2016 to December 31, 2023 were collected. Strains were conducted resistance analysis and serotype testing. Results Among the 163 GBS strains, 121 and 42 were invasive and non-invasive infection isolates, respectively. No strains were found to be resistant to penicillin, ceftriaxone, cefepime, linezolid, and vancomycin, and resistance rates to erythromycin, clindamycin, and levofloxacin were 91.4%, 90.8%, and 53.4%, respectively. Non-invasive infection isolates had a higher resistance rate to levofloxacin than invasive infection isolates. The distribution of bacterial serotypes from high to low was as follows: type Ⅰb (n=75, 46.0%), type Ⅲ (n=65, 39.9%), type Ⅴ (n=13, 8.0%), type Ⅰa (n=6, 3.7%), type Ⅱ (n=2, 1.2%), type Ⅳ and Ⅵ (n=1, 0.6%, each). There was a statistically significant difference in the distribution of serotypes between invasive and non-invasive infection isolates (P<0.05). Serotype distributions of erythromycin- and clindamycin-resistant GBS strains were both statistically different between two groups (both P<0.05), while serotype distribution of levoflo-xacin-resistant GBS strains was not statistically different between two groups (P>0.05). Conclusion GBS strains in children in Beijing have high resistance rates to erythromycin and clindamycin, with serotypes Ⅰb and Ⅲ being more frequent. Serotypes with high prevalence have higher resistance. Continuously monitoring on the epidemiology of GBS infection is crucial for the clinical prevention and treatment of GBS infection in children as well as the development of vaccines.