Objective To analyze the status and gene subtype distribution of human papillomavirus (HPV) infection in male patients in dermatology outpatient department, provide reference for the prevention and treatment of male HPV infection. Methods Male patients who visited and conducted HPV detection in the dermatology outpatient department of a hospital from January 2022 to March 2023 were retrospectively surveyed. Patients were divi-ded into five groups: viral warts group, dermatitis and rash group, urinary tract infection group, balanoposthitis group, and asymptomatic group. Relationship between genotype distribution and patient age, clinical diagnosis, and symptom types was statistically analyzed. Results A total of 1 035 male patients underwent HPV detection, out of which 567 were positive, with a positive detection rate of 54.78%. 286, 164, 6, 109, and 470 cases were from viral warts, dermatitis and rash, urinary tract infection, balanoposthitis, and asymptomatic group, respectively. 21 subtypes of HPV were detected, with the top three subtypes being type 6(17.97%), 11(12.37%), and 52(8.70%). The positive rate of single type HPV infection was 29.86%, accounting for 54.50%. Positive rates of infection, low-risk infection, and multiple mixed infection in different age groups were compared, differences were all statistically significant (all P<0.05). The positive infection rate in the age group of <20 years old was higher than that in the age groups of 20-<30, 30-<40, and 40-<50 years old, differences were all statistically significant (all P<0.05). Among the positive patients, 199 cases (35.10%) had no clinical symptoms, while 368 (64.90%) had clinical symptoms, mainly manifested as viral warts (40.74%, n=231). In viral warts group, HPV-positive patients were mainly of low-risk type, accounting for 80.95%; In balanoposthitis group, HPV-positive patients were mainly of high-risk type, accounting for 84.78%; In asymptomatic group, HPV-positive patients were mainly infected with high-risk types, accounting for 86.43%. Conclusion HPV infection in male outpatient department of dermatology is mainly single type infection. The clinical diagnosis of low-risk infection is mainly viral warts, while high-risk infection is mainly manifested as balanoposthitis. In asymptomatic group, positive infections are mainly of high-risk type.