Objective To evaluate the disinfection quality of gastroscopes and enteroscopes in medical institutions across China, and provide scientific guidance for the disinfection of digestive endoscopes. Methods From 2010 to 2022, relying on the "National Hospital Disinfection and Infection Control Surveillance" project, the disinfection status of gastroscopes and enteroscopes in 184 medical institutions in 30 provinces in China was investigated, clea-ning and disinfection quality of gastroscopes and enteroscopes was analyzed. Results From 2010 to 2022, a total of 6 421 post-disinfection endoscope specimens were collected from 184 medical institutions, including 4 011 gastroscope specimens and 2 410 enteroscope specimens. From 2010 to 2022, the qualified rate of disinfection of gastroscopes and enteroscopes in China increased from 87.6% to 97.9% and 75.9% to 95.4%, respectively. The average qualified rate of disinfection of endoscopes in secondary medical institutions was lower than that in tertiary medical institutions (87.5% vs 92.1%). The disinfection efficacy of different kinds of endoscopic disinfectants was also different, among which the qualified rate of peracetic acid was the highest (95.6%-95.7%), followed by o-phthalaldehyde (93.6%-95.0%). The qualified rate of acidic electrolyzed-oxidizing water was low, only 55.0%-85.0%. Conclusion The disinfection quality of gastroscopes and enteroscopes in China has been continuously improved, and it is still necessary to strengthen the surveillance on daily disinfection quality.