Objective To systematically evaluate the effectiveness of active screening in the prevention and control of healthcare-associated infection (HAI) with carbapenem-resistant Enterobacterales (CRE) in Chinese medical institutions through Meta-analysis. Methods Studies on active screening combined with intervention for CRE in Chinese medical institutions published from the establishment of databases to April 2024 were retrieved from Wanfang Database, VIP database, China National Knowledge Infrastructure, China Biomedical Literature Database, Embase, PubMed, Cochrane Library, and Web of Science. Meta-analysis was performed to study the effectiveness of active screening combined with intervention in CRE prevention and control. Results A total of 14 literatures were included in the analysis, including 12 non-randomized controlled studies and 2 randomized controlled studies. Meta-analysis results showed that active screening and timely intervention measures against CRE in hospitalized patients could effectively reduce the incidence of HAI with CRE (relative risk ［RR］=0.51, 95%CI ［0.43, 0.61］, P<0.05). Conclusion Active screening combined with intervention for CRE among hospitalized patients can effectively reduce the risk of CRE cross infection in hospitals.