Objective To investigate a cluster of healthcare-associated infection (HAI) of carbapenem-resistant Klebsiella pneumoniae (CRKP) in intensive care unit (ICU) of a traditional Chinese medicine hospital, and provide evidence for the prevention and control of multidrug-resistant organisms in hospitals. Methods From January to July 2023, multiple cases of CRKP infection in the ICU of a traditional Chinese medicine hospital occurred, surpassing the number in the same period of previous years. Through collection of patients’ clinical information, epidemiological investigation and environmental hygiene monitoring, the isolated CRKP strains were identified by pulsed-field gel electrophoresis (PFGE), possible causes of transmission were found out, and corresponding control measures were taken. Results During the study period, a total of 13 patients were confirmed to have developed CRKP infection, mainly respiratory tract infection, bloodstream infection, and abdominal infection. Environmental monitoring showed that only 2 out of 131 environmental object surfaces specimens and staff’s hand specimens tested positive for CRKP. PFGE identification showed that the 2 CRKP strains isolated from the sink specimens were highly homologous to the strains isolated from 2 recently admitted patients. Some strains isolated from patients were highly homologous, indicating a possibility of cross transmission. Conclusion CRKP is prone to be cross-transmission among ICU patients and contaminates the environment. Early warning and investigation should be conducted to prevent the occurrence of HAI outbreaks.