Objective To investigate the current status of job competency of full-time personnel who engaged in gastrointestinal endoscope cleaning and disinfection in medical institutions in Hubei Province, analyze the influencing factors, and provide reference for conducting the training for full-time personnel in gastrointestinal endoscope clea-ning and disinfection. Methods Through expert consultation and revision, as well as forming of questionnaire, an online survey was conducted. The questionnaire was filled out by full-time personnel who engaged in endoscope cleaning and disinfection in the endoscopy centers/rooms in medical institutions. Results A total of 195 full-time personnel engaged in cleaning and disinfecting gastrointestinal endoscopes in 185 medical institutions participated in the survey, with a self-assessment score of (4.48±0.41) point for job competency. Univariate analysis showed that there were statistically significant differences in the self-assessment scores of job competency based on different le-vels of training, training frequency, income satisfaction, and daily diagnosis and treatment volume (all P<0.05). Multiple linear regression analysis indicated that full-time personnel with working years ＞10 years, satisfying with their income, participating in provincial/national level training, receiving training at least once per year, and having an average daily diagnosis and treatment volume >50 cases had higher self-assessment score of job competency (all P<0.05). Conclusion In order to enhance the job competency of personnel who engage in gastrointestinal endoscope cleaning and disinfection, it is recommended to encourage the participation in provincial/national training, and the hospital-wide training should emphasize the application and disposal of cleaning, disinfection, and storage devices of gastrointestinal endoscope, as well as disinfection knowledge, and encourage personnel to conduct relevant research.