Objective To explore the application effect of constructing a data sharing-based multidrug-resistant organism (MDRO) information reminder system combined with multidisciplinary standardized measures on the whole-process closed-loop management of MDRO. Methods Hospitalized patients from whom MDRO were detected from January 1 to July 31, 2024 were taken as the control group, those from August 1, 2024, to May 31, 2025 were as the intervention group. The control group adopted the traditional manual reporting mode for MDRO, while the intervention group adopted the combination of MDRO reminder system and multidisciplinary management mode. Differences in isolation order issuing rate for MDRO and process- and outcome-indicators for multidisciplinary specialty before and after system implementation were compared. Results After implementing information reminder system combined with multidisciplinary measures, issuing rates of ≤24 hour and ＜4 hour MDRO isolation orders in the intervention group were both higher than in the control group ([90.20% vs 65.23%, P<0.05],[80.64% vs 55.08%, P<0.001], respectively). Antimicrobial use rate in the intervention group was lower than in the control group (36.27% vs 43.41%), with a statistically significant difference (P<0.05). The implementation rates of MDRO prevention and control measures, pathogen detection rates, and fluorescence labeling clearance rates in the intervention group were all higher than those in the control group, all with statistically significant differences (all P<0.05). The detection rate of MDRO decreased compared with the control group (10.83% vs 16.49%), and the incidence of MDRO healthcare-associated infection in the intervention group was lower than that in the control group (0.09% vs 0.19%), both with statistically significant differences (both P<0.001). The treatment expense in the intervention group reduced by 27 422 Yuan compared with the control group. Conclusion This study constructed an MDRO reminder system, realized full-chain monitoring system for the multidisciplinary MDRO information interaction platform, strengthened multidisciplinary in-depth integration, and improved the prevention and control effect for MDRO.