Objective To investigate the feasibility of safe injection measures in clinical practice, provide basis for the formulation of industry standards for "Standard for safe injection", improve standards, and further strengthen the safe injection-related work of healthcare workers (HCWs). Methods Based on the contents of the "Standard for safe injection", a self-designed questionnaire was formulated. From July 13, 2023 to August 13, 2023, conve-nience sampling method was conducted to survey voluntarily participated doctors, nurses, anesthesiologists, pharmacists, technicians, rehabilitation therapists, and administrative personnel from various provinces across the country. Results A total of 28 924 valid questionnaires were collected from 29 provinces/municipalities/autonomous regions. 17 524 persons (60.59%) answered correctly regarding the definition of safe injection. The correct answering rates of HCWs of different regions, hospital levels, occupational titles, ages, years of service, and positions all showed statistically significant differences (all P<0.05). Among the 47 survey items, HCWs’ selection for "completely achievable" was as follows: more than 90% selected 20 items, 80%-89% selected 22 items, and less than 80% selected 5 items, mainly including hand hygiene, surface cleaning of items and equipment, use of protective equipment, and use of blood glucose meters. There were all statistically significant difference in the proportion of HCWs in different positions who judged these 5 items as "completely achievable"(all P<0.05). Conclusion HCWs understand on the definititon of safe injection to some extent, and generally believe that safe injection measures are highly feasible. However, based on the actual situation of clinical practice nationwide, the implementation of safety injection measures still needs to be further standardized and improved.