ObjectiveTo investigate the awareness of tuberculosis(TB)related knowledge and the status of occupational protection among health care workers (HCWs) in a general hospital.MethodsQuestionnaires were used to investigate the awareness of TBrelated knowledge and the status of occupational protection among HCWs.ResultsA total of 333 HCWs were enrolled in the investigation, correct response rate in the TBrelated knowledge was 62.84%-93.09%; correct response rate in national TBrelated policy was 59.52%-77.27%.The implementation rate of protective measures was not significantly different between the awareness group and the unawareness group (［10.74%-79.34%］ vs ［10.38%-82.55%］,P>0.05), but implementation rate of regular medical examination in awareness group was significantly higher than unawareness group（92.56% vs 79.25%， P＜0.05）.ConclusionThe knowledge about tuberculosis and occupational protection training should be strengthened among HCWs in general hospitals.