ObjectiveTo analyze the distribution and drug  resistance of pathogens isolated from patients in intensive care units( ICUs) and  nonICUs, so as to provide evidence for clinical antiinfective treatment. MethodsBacterial  strains from patients in clinical departments from May 2006 to May 2007 were isolated and identified,  pathogenic distribution and antimicrobial resistance  between ICUs and nonICUs were compared and analyzed.ResultsOne hundred and fifty strains were isolated from patients in ICUs, the constitutional ratio of nonfermentative bacteria was 40.67%(61/150); 495 strains were isolated from nonICUs, the constitutional ratio of nonfermentative bacteria  was 17.98%(89/495). The isolation rate of nonfermentative bacteria from ICUs was higher than that of nonICUs (χ2=33.20，P<0.01). The most common pathogens in ICUs were fungi（21.33％），Pseudomonas aeruginosa（17.33％） and Escherichia coli（12.67％）; The most common pathogens in nonICU were fungi (20.61%)，Escherichia coli (17.98%) and Staphylococcus aureus (10.30%). Antimicrobial resistance of Acinetobacter baumannii and Staphylococcus aureus isolated from ICUs were serious;  The resistance to the third generation cephalosporins for treating infection with gramnegative bacilli was quite different between ICUs and nonICUs.ConclusionThe isolation rate of nonfermentative bacteria in ICUs is high, and drug resistance is serious, which should be given strengthened management and surveillance.