ObjectiveTo evaluate the inhibitory activities of deoxynojirimycin (DNJ) and its derivate Nbutyldeoxynojirimycin (NbutylDNJ) against hepatitis B virus(HBV) in vitro. MethodsHepG2 2.2.15 cell were treated with DNJ. At 3rd, 6th and 9th day after treatment, the supernatant was collected for HBsAg, HBeAg and HBV DNA quantitative assay. ResultsAt 9th day of incubation, 1 000 μg/mL DNJ and 100 μg/mL NbutylDNJ had obvious inhibitory effect on the replication of virus, there were significant differences compared with control group (both P＝0.001). ConclusionDNJ and its derivate have no obvious and direct cytotoxicity effect at the experimental concentration, 1 000 μg/mL DNJ and 100 μg/mL NbutylDNJ can inhibit the replication of HBV in HepG2 2.2.15 cell.