ObjectiveTo study the qacE△1sul1 gene in multiply drugresistant Pseudomonas aeruginosa. MethodsThe susceptibility of 30 strains of clinical isolated Pseudomonas aeruginosa to 15 antimicrobial agents was tested by KirbyBauer method，and qacE△1sul1 gene was analyzed by PCR.ResultsIn 30 strains of Pseudomonas aeruginosa, the positive rate of qacE△1sul1 gene was 43.33%(13/30).ConclusionThere is higher positive rate of qacE△1sul1 gene in Pseudomonas aeruginosa isolated from the inpatients, it is reported for the first time in Shandong provine.