ObjectiveTo study antimicrobial prophylactic usage in clean surgical operations during perioperative period, so as to strengthen the management of antimicrobial usage．MethodsPerioperative antimicrobial prophylactic use for 177 clean surgical operations were investigated retrospectively and statistically analyzed．ResultsAmong all cases，165 cases(93.22%) were given antimicrobial agents 0.5～2 hours before the operations;162 (91.53%) cases were administrated by intravenous drip. Among 56 patients whose duration of operation was longer than 3 hours, 3 patients(5.36%) received an additional intraoperative antimicrobial dose. Singledose antimicrobial prophylaxis was given in 25 cases（14.12%）; 67 cases（37.85%） received antimicrobial prophylaxis for 3~7 days after operations; 42 cases (23.73%) received antimicrobial prophylaxis for more than 7 days and 20 (11.30%) for more than 48 hours. 20 kinds of 7 categories of antimicrobial agents were used in the operations, 52.71% were cephalosporins (the majority were the first and second generation cephalosporins), followed by penicillins(28.08%). Drugs were used mainly in single dosage. ConclusionInappropriate usage was found in antimicrobial prophylaxis for clean surgical operations during perioperative period. Education about antimicrobial prophylaxis in perioperation to surgeons must be strengthened and management must be enhanced.