ObjectiveTo investigate the detection and change in drugresistance of  extendedspectrum βlactamase (ESBLs)producing Escherichia coli, so as to provide reference for the rational use of antimicrobial agents in clinic.MethodsEscherichia coli isolated from clinic in a hospital between January~December, 2006 were collected, the detection of ESBLs  and drugresistance were compared with those of 2002. ResultsThe rate of ESBLsproducing Escherichia coli isolated in 2006 was 65.54% (175/267), which increased obviously compared with 47.92% (46/96) isolated  in 2002 (χ2=9.21, P<0.05). The susceptibility rate of ESBLsproducing Escherichia coli to imipenem in 2006 was 24.00%, which decreased obviously compared with 52.17% in 2002 (χ2=13.80, P<0.05); The susceptibility rates to the other antimicrobial agents had no statistic significance, imipenem still had ideal activity against ESBLsproducing Escherichia coli. ConclusionThere is increasing tendency in the detection of ESBLsproducing Escherichia coli, and drugresistance is high, which should be paid attention.