ObjectiveTo investigate drugresistance and multiple drugresistant genes  of Enterobacter cloacae (E.cloacae) in nosocomial infection(NI), so as to  provide evidence for the  treatment of IN.MethodsForty trains of  E.cloacae isolated from clinic were performed antimicrobial susceptibility test by disc agar diffusion and agar dilution method, twelve kinds of antimicrobialresistant genes were detected by polymerase chain reaction(PCR) and DNA sequencing. ResultsForty strains of E. cloacae were only highly sensitive to imipenem and meropenem, sensitive rate were all 100.00%;the resistant rate to cefepime was low(15.00%); and resistant rates to the other 15 kinds of antimicrobial agents were high (42.00%~92.50%). Eight kinds of drugresistant genes were detected, which was TEM1,SHV2a,CTXM3, CTXM9, AmpC,aac(3′)Ⅰ,IntⅠ1 and sul1,sul1+ IntⅠ1 was the main pattern; drugresistant pattern was divided into A~I 9, the main pattern were C and D.ConclusionE. cloacae shows a multiple  and high drugresistance, resistant mechanisms are complicated and varied.