To evaluate in vitro activity of linezolid and other antimicrobial agents against Enterococci spp. isolated from clinic.MethodsIdentification and  antimicrobial sensitivity test of Enterococci spp. was carried out by Microscan WalkAway40 system.ResultsNo  linezolidresistant strain was detected in 117 Enterococci spp. strains. Resistant rate of Enterococcus faecium and Enterococcus faecalis against vancomycin was 4.41% and 5.71% respectively, the resistant rates to  penicillin, ampicillin, erythromycin, high concentration  gentamycin, levofloxacin  and ciprofloxacin were all above 70%.ConclusionLinezolid has  high activity against Enterococci spp., especially the control of infections caused by multidrugresistant  isolates.