realize the detection and drug resistance of nonfermentative bacteria from clinical samples between January and December, 2008, so as to provide evidence for rational use of antimicrobial agents in clinic. MethodsBacteria were identified by ATB identification system, antimicrobial susceptibility tests were performed by KirbyBauer method. Results215 strains of nonfermentative bacteria were isolated from 3 555 samples, the isolation rate was 6.05%, which accounting for 19.15% (215/1 123) of total isolated bacteria; the most common isolated bacteria was Pseudomonas aeruginosa, which accounting for 41.86% of total nonfermentative bacteria, the next was Acinetobacter baumannii and Burkholderia cepacia, the constitutional ratio was 29.30% and 16.74% respectively. The isolation rates of nonfermentative bacteria varied with different infection sites, the isolation rates were high in  samples of sputum and throat swabs, and excretion of subcutaneous tissue, which was 15.32% and 9.17% respectively. Pseudomonas aeruginosa, Acinetobacter baumannii, Burkholderia cepacia and Stenotrophomona maltophilia were all had obvious drug resistance. ConclusionThe isolation rate of nonfermentative bacteria in clinical infected patients are high, and drugresistance is strong. In order to control nonfermentative bacteria infection and  emergence of drug resistant strain, antimicrobial agents for treatment of infection should be chosen according to antimicrobial susceptibility test results.