Objective To analyze the epidemiological characteristics of methicillin resistant Staphylococcus aureus (MRSA) in nosocomial infection with pulsed field gel electrophoresis (PFGE) and PCR SCCmec typing.Methods Forty strains  of MRSA  collected from Tianjin Nankai Hospital between January, 2007 and December, 2008  were performed  antimicrobial susceptibility analysis, PFGE and SCCmec genotyping.Results Antimicrobial susceptibility patterns of  40 strains of MRSA were highly similar,  isolates were classified into AE five types by PFGE typing，type A, B, C, D, E were 21, 8, 4, 6, 1 strains respectively； SCCmec typing showed that 1 strain was SCCmecⅠ,33 were SCCmecⅢ, 1 was SCCmecⅣ，and 5 were  SCCmecV. Sporadic infection of MRSA occurred in the hospital during the studied period. Conclusion Epidemiological analysis indicates that PFGE typing can identify the genetic relationship between isolates, SCCmec typing can  suggest whether  MRSA isolates are hospitalacquired .Combination of  two genotyping methods can show characteristics of epidemic strains, and provide reference for nosocomial infection surveillance.

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