ObjectiveTo understand the pathogenic characteristics and drug sensitivity of 8 strains of Neisseria meningitidis isolated in Hunan province between 2007—2008.MethodsNeisseria meningitidis strains were identified and clustered by serology and PCR test, drug sensitivity test was carried by minimum inhibitory concentration (MIC) agar dilution method.ResultsAmong  8 strains of Neisseria meningitidis, 5 strains were in serogroup C, 1 in serogroup B, 1 in serogroup Y and 1 was unidentified. All 8 strains were sensitive to penicillin, ampicillin, minocycline, ceftriaxone, cefotaxime, meropenem, rifampicin, but resistant to sulfamethoxazole/trimethoprim,  4 strains were resistant to ciprofloxacin and levofloxacin respectively, and 1 strain was resistant to chloramphenicol and azithromycin respectively.ConclusionEight epidemic strains of Neisseria meningitidis are mainly in serogroup C; penicillin, ceftriaxone, cefotaxime and meropenem should be used as the firstline drugs for the treatment of epidemic cerebrospinal meningitis, the resistance to quinolones and macrolides should be paid attention.