ObjectiveTo explore the distribution of disinfectantsulfadiazineresistance gene qacEΔ1sul1 in Pseudomonas aeruginosa (P. aeruginosa) and the resistance of positive strains to four kinds of disinfectants (iodophor, glutaraldehyde, chlorhexidine and “84” disinfectant ).MethodsTwenty strains of P.aeruginosa isolated from a hosptial between December, 2007 and January 2008 were detected qacEΔ1sul1 gene by polymerase chain reaction, minimum inhibitory concentrations (MICs) of the above four disinfectants were detected.ResultsIn 20 strains of P. aeruginosa, 10(50.00%) strains were positive for qacEΔ1sul1 gene. MICs of iodophor, glutaraldehyde, chlorhexidine and “84” disinfectant to positive qacEΔ1sul1 strains were 8.0~128.0 μg/mL,16.0~256.0 μg/mL，1.0~16.0 μg/mL， and 4.0~64.0  μg/mL, respectively.ConclusionqacEΔ1sul1 gene is widespread in the clinical isolated P. aeruginosa, there are differences in resistance of positive strains against four kinds of disinfectants, disinfectants should be used rationally so as to control the spread of  P. aeruginosa.