ObjectiveTo investigate fungal infection and drug resistance of fungi in ICU patients with external intubation.Methods161 specimens of intubator used by 84 ICU patients in a hospital were collected, and specimens were inoculated on Candidal chromogenic medium , all strains were identified by API 20C AUX Kit,and drug sensitivity test were performed by ATB FUN3 Kit.ResultsNinetyeight (60.87%) fungus strains were isolated from 161 specimens, most of which were Candida albicans (52, 53.06%), the next were Candida tropicalis (14, 14.29%), Candida glabrata (11, 11.22%), Candida parapsilosis (9, 9.18%) , and Candida krusei (12, 12.25%). Sixty strains (61.22%) were isolated from respiratory intubator, 25(25.51%) from urethral catheter and 13(13.27%) from venous cannula. Drugresistant rate of isolated strains to 5fluorocytosine, amphotericin B, and voriconazole were all <10% , resistant rate to itraconazloe was 12.24%;resistant rate of Candida glabrata, Candida parapsilosis and Candida krusei to fluconazol were all >45%.ConclusionCandida albicans is the main fungi in infection caused by external intubation; fungal infection rate in patients undergoing respiratory intubation is high than patients undergoing the other external intubation ; All kinds of Candida have low drugresistance to 5fluorouracil, amphotericin B and fluconazole and have high drugresistance to fluconazole.