ObjectiveTo report the clinical and laboratory detection data of 4 cases of rare deep infections caused by Sporothrix schenckii, Fusarium moniliforme, Penicillium marneffei and Scedosporum apiospermum. MethodsSecretion and blood specimens from patients' infected sites were taken for direct smear and culture , fungi were identified according to colony features and appearance under microscope. ResultsFour strains of filamentous fungi isolated from the wound secretion and blood specimens were Sporothrix schenckii, Fusarium moniliforme, Penicillium marneffei and Scedosporum apiospermum .ConclusionInfection caused by Sporothrix schenckii, Fusarium moniliforme, Penicillium marneffei and Scedosporum apiospermum in clinic are very rare, and the detection is also difficult. The knowledge and laboratory detection on the rare fungi infection should be strengthened to improve the level of diagnosis and therapy.