ObjectiveTo investigate  the disinfectantresistant gene qacE△1sul1 in clinical multidrugresistant (MDR) gramnegative bacilli.MethodsPolymerase chain reaction (PCR) was used to detect disinfectantresistant gene qacE△1sul1 in 235 isolates from clinic，including 155 multidrugresistant and 80 antimicrobial susceptible isolates. PCR products were sequenced by DNA sequencing.ResultsAmong 50 strains of  ESBLsproducing Escherichia coli, 27 ESBLsproducing Klebsiella pneumoniae, 50 MDR Pseudomonas aeruginosa, and  28  MDR Acinetobacter baumannii，disinfectantresistant gene qacE△1sul1 were detected in 37（74.00%）,24（88.89%）,46（92.00%）and 28 strains（100.00%）,respectively， the total detection rate was 87.10%; Among nonESBLsproducing antimicrobial susceptible isolates of Escherichia coli,Klebsiella pneumoniae,Pseudomonas aeruginosa, and Acinetobacter baumannii （each were 20 isolates），disinfectantresistant gene qacE△1sul1 were detected in 16（80.00%）,7（35.00%）,5 （25.00%）and 13 strains（65.00%）,respectively, the total detection rate of qacE△1sul1 gene was 51.25％.ConclusionDisinfectantresistant gene qacE△1sul1 has a high detection rate in clinical MDR gramnegative bacilli in this hospital, it is important to the rational use of disinfectant by  intensifying  the monitor on the disinfectantresistance of MDR .