The relationship between hepatitis B virus mutation at nucleotides 1653T and liver cancer
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R512.6+2

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    Abstract:

    ObjectiveTo study the relationship between hepatitis B virus (HBV) mutation at nucleotides 1653T and liver cancer. MethodsOne hundred and nineteen serum samples of HBVinfected patients were collected (All were positive HBV DNA) , 24 ,35, 29, and 31samples were from asymptomatic carriers(ASC), patients with chronic hepatitis B (CHB), liver cirrhosis (LC) ,and hepatocellular carcinoma (HCC),respectively. Partial fragments of HBV pre C and C gene were amplified by seminested polymerase chain reaction . The PCR products were subjected to direct sequencing and the mutation at 1653T was determined by sequence analysis. HBV genotypes were detected by restriction fragment length polymorphism based on S gene PCR products. ResultsAll samples from 24 ASC were not found mutation at nucleotides 1653T ; 1(2.86%) sample from 35 CHB and 5(17.24%) from 29 LC were found mutation at 1653T; The detection rate of 1653T mutation was not significantly different between CHB and LC(χ2=2.36, P>0.05);14(45.16%) samples were found mutation at 1653T in HCC,the mutation at 1653T in HCC was significantly higher than that of LC(χ2=5.40,P<0.05). Of 119 serum samples of HBV, 2 of 51 genotype B and 18 of 58 genotype C were detected mutation at nucleotides 1653T , the other 10 samples couldn’t be determined genotype .The difference in mutation at 1653T between genotype C and B was significant (χ2=11.57, P<0.01).ConclusionHBV 1653T mutation is correlated with HCC, and is more prone to occur in genotype C infection; 1653T mutation can be as predictive risk factors for hepatocarcinogenesis.

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刘悦晖 ,丁静娟.乙型肝炎病毒1653T变异与肝癌相关性研究[J].中国感染控制杂志英文版,2011,10(4):260-262.
LIU Yuehui, DING Jingjuan. The relationship between hepatitis B virus mutation at nucleotides 1653T and liver cancer[J]. Chin J Infect Control, 2011,10(4):260-262.

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History
  • Received:March 28,2010
  • Revised:June 22,2010
  • Adopted:
  • Online: July 30,2011
  • Published: