ObjectiveTo investigate drug resistance and antimicrobial combination against 38 strains of methicillinresistant Staphylococcu aureus (MRSA) isolated from intensive care unit (ICU) patients, so as to select rational antimicrobial agents to treat MRSA infection.MethodsThe isolates were identified by VITEK2 automatic bacterial identification system and Staphylococcus aureus latex coagulation method, MRSA were detected with Staphylococcus aureus latex coagulation method. Drug susceptibility tests were performed with microbroth dilution method based on Clinical and Laboratory Standards Institute (CLSI) Standard .ResultsThirtyeight MRSA stains were sensitive to linezolid (LZD), vancomycin (VAN), and teicoplanin (TEC)； the resistant rate to levofloxacin (LVX) and rifampicin (RIF) was 100.00% and 78.95% respectively. The fractional inhibitional concentration (FIC)<1 of RIF combining with LZD,VAN and TEC was 13.16%-31.58%; The FIC<1 of LVX combining with LZD,VAN and TEC was 2.63%-36.84%， the difference between antimicrobial combination had statistical significance （P<0.01）. ConclusionRational choice of antimicrobial agents to treat MRSA infection in ICU patients should be according to antimicrobial agents and patients’ state.