ObjectiveTo study the distribution and drugresistance of methicillinresistant Staphylococcus aureus (MRSA), so as to provide reference for rational use of antimicrobial agents in clinic.MethodsData of 756 Staphylococcus isolates from clinical samples in a hospital from May 2008 to May 2010 were analyzed retrospectively.ResultsOf all Staphylococcus, 584 (77.25%) were Staphylococcus aureus, 394 (67.47%) of which were MRSA.MRSA mainly came from respiratory tract (248 isolates, 62.94%) and wound (121 isolates, 30.71%), and were mainly from intensive care unit (ICU) (53.30%) and surgery patients (19.29%). MRSA were all sensitive to vancomycin, teicoplain, and linezolid, but resistant to the other commonly used antimicrobial agents,minimum inhibitory concentration of 5.33% of MRSA to vancomycin was 2 μg /mL.ConclusionMRSA are mainly isolated from respiratory tract and wound of ICU and surgery patients, infection and drug resistance is serious, and has multiple drug resistance ; detection and drug resistant surveillance of MRSA should be strengthened.