ObjectiveTo study the distribution and drug resistance of coagulase negative Staphylococcus (CNS) isolated from the object surface and hands of healthcare workers in medical settings, and to provide scientific basis for controlling healthcareassociated infection.MethodsCNS were isolated and identified with bacterial biochemical identification instrument WalkaWay40s1 and microbiochemical tube, antimicrobial susceptibility testing was performed with disk diffusion method, drugresistant gene mecA was detected by polymerase chain reaction method.ResultsA total of 63 isolates of CNS were isolated from 478 object surface samples and 363 hand samples, the isolation rate was 7.49%, 15 CNS isolates were from object surface, 38 were from nurses’ hands and 10 were from doctors’ hands. 24 (38.09%) isolates carried mecA gene and were methicillinresistant CNS (MRCNS),12 of which were Staphylococcus epidermidis, 6 were Staphylococcus haemolyticus, 5 were Staphylococcus warneri，and 1 was Staphylococcus capitis subsp.capitis; drug resistant rate of Staphylococcus epidermidis, Staphylococcus haemolyticus, and Staphylococcus warneri to penicillin, amoxicillin, ampicillin/sulbactam, erythromycin, cefazolin, and imipenem was above 87.50% respectively, the resistant rate to trimethoprim/sulfamethoxazole， levofloxacin, clindamycin,ciprofloxacin,tetracycline, and gentamycin were 20.83%-45.83%. ConclusionCNS carried by object surface and hands of healthcare workers in medical settings are multidrugresistant and should be paid attention.