ObjectiveTo identify and characterize one Enterobacteriaceae isolate which could crossagglutinate with Salmonella diagnostic antiserum.MethodsPhenotype and genotype of the isolate were determined by culture，metabolic assay, agglutination reaction， and 16S rRNA sequence phylogenetic analysis, respectively.ResultsThe isolate was consistent with Escherichia coli on culture and biochemical features, but could crossagglutinate with multiple types or groupspecific Salmonella antiserum rather than Escherichia coli antiserum. 16S rRNA sequence analysis revealed that the isolate was identical with Escherichia coli, but was obviously different from Salmonella spp.ConclusionOne Escherichia coli isolate which can crossagglutinate with Salmonella diagnostic antiserum has been obtained in this study.