ObjectiveTo investigate the resistance of clinical bacterial isolates from a hospitals in 2010.MethodsAntimicrobial susceptibility of bacterial isolates from inpatients was detected by BDautomatic identification of bacterial analyzer, and data were analyzed with WHONET5.4 software.ResultsA total of 2 192 pathogenic strains were isolated from various clinical specimens from January to December，  2010, 67.97% of which was gramnegative bacilli and 32.03% was grampositive cocci. 19.69% of Staphylococcus aureus and 54.59% of coagulase negative Staphylococcus was methicillinresistant (MRSA and MRCNS respectively). The resistant rates of methicillinresistant strains to βlactams and other antimicrobial agents were much higher than those of methicillinsensitive strains. The resistant rate of MRSA to sulfamethoxazole/trimethoprim, rifampin, tetracycline, and gentamicin was 1.67%, 41.54%,44.62%,and 58.46%， respectively, the resistant rates of MRCNS to rifampin and tetracycline was 17.27% and 36.70%， respectively; vancomycin,teicoplanin and linezolidresistant strain was not found. The resistant rates of Enterococcus faecalis to most detected antimicrobial agents were much lower than those of Enterococcus faecium. One linezolidresistant Enterococcus faecalis isolate was first reported in this hospital, vancomycinresistant strain was not found. Extendedspectrum βlactamase(ESBL)producing strains accounted for 44.29% of Escherichia coli and 15.79% of Klebsiella pneumoniae. Resistant rates of ESBLproducing Enterobacteriaceae strains were higher than nonESBLproducing Enterobacteriaceae strains. Resistant rate of Pseudomonas aeruginosa to imipenem and meropenem was 26.73% and 13.79%, respectively, resistant rate of Acinetobacter spp. (92.91% were Acinetobacter baumannii) to above two carbapenems was 31.35% and 27.17%, respectively.ConclusionBacterial resistance is on the rise, especially drug resistance of gramnegative bacilli; resistance of Acinetobacter baumannii and Pseudomonas aeruginosa to carbapenems is increasing.It is important to use antimicrobial agents rationally, detect pandrugresistant strains early, and strengthen infection control.