ObjectiveTo investigate the occurrence of occupational exposure to bloodborne pathogens, and put forward preventive measures.Methods135 cases of occupational exposure to bloodborne pathogens among  healthcare workers (HCWs) in two hospitals from January 2008 to December 2010 were investigated and analyzed.ResultsThe top 3 exposure rates were observed among nurses (54.81%), doctors (25.19%) and housekeeping staff (16.30%); sharp injuries were the most common causes (91.11%), which occurred frequently during needlewithdrawing and suturing; among bloodborne pathogens, hepatitis B virus, hepatitis C virus, human immunodeficiency virus, and Treponema pallidum accounted for 20.18%,  2.63%,  2.63% and 6.14% respectively. None of HCWs was infected after taking proper preventive measures following exposure.ConclusionStrengthening occupational safety education, carrying out standard prevention, standardizing manipulation, local treating for injured site correctly, and reporting the occurrence of injury timely following exposure can  reduce the risk of occupational infection with bloodborne pathogens.