ObjectiveTo study the effect of  monitoringtrainingplanning(MTP) intervention approach on perioperative prophylactic antimicrobial use in classⅠ incision surgery. MethodsIn accordance with the Clinical Antimicrobial Application Supervision  Scheme in China in 2011 and rational drug selection indicators of  World Health Organization ,  the case histories of patients receiving classⅠ incision operation in department of general surgery in the first  quarter of  2011 were selected  as the baseline, and MTP  intervention was then carried out，during the next three quarters, investigated  indicators were analyzed . In this way, three cycles of MTP intervention investigation were implemented.ResultsAfter three cycles of interventions, rate of perioperative prophylactic antimicrobial use , average drug exposure days , and rate of combination of antimicrobial agents  decreased  from 100.00%, (1.65±0.80) days and 15.00% to 26.32%, (0.26±0.46) days and 0.00% (All P<0.05) respectively; preoperative antimicrobial use rate (0.5-2 h before operation) and  rational drug selection rate both reached 100.00%. ConclusionMTP intervention mode is feasible and effective in promoting the rational use of antimicrobial agents for perioperative prophylaxis in classⅠ incision surgery.