ObjectiveTo realize the detection and drug resistance of βlactamaseproducing Staphylococcus isolated from clinical specimens in a hospital, so as to provide reference for preventing and treating Staphylococcus infection.MethodsClinical and laboratory data of 1 725 βlactamaseproducing Staphylococcus isolated from clinical sepcimens between 2005 and 2010 were analyzed retrospectively.ResultsThe total detection rate of βlactamaseproducing Staphylococcus was 94.83% (1 725/1 819). The detection rate of each year was 88.68%（94/106）, 89.69%（174/194）, 91.72%（155/169）,97.58%（242/248）,96.10%（591/615） and 96.30%（469/487） respectively，there was an increased tendency（χ2=51.11, P<0.01; The detection rate of βlactamase was the highest in Staphylococcus sciuri（99.50%，198/199） and the lowest in Staphylococcus auricularis (80.37%，86/107）（χ2＝58.08，P<0.01）. Antimicrobial susceptibility testing results showed that βlactamaseproducing Staphylococcus isolates were sensitive to vancomycin, linezolid, furantoin and rifampicin, but were highly and multiply resistant to penicillin, ampicillin, ampicillin/sulbactam, oxacillin, cefazolin, ciprofloxacin, tetracycline, clindamycin, erythromycin, gentamicin and sulfamethoxazole/trimethoprim, the resistant rate was between 51.74% and 100.00%, and resistant rate of βlactamaseproducing Staphylococcus was higher than nonβlactamaseproducing Staphylococcus (χ2=7.00-485.39. P<0.01). 72.86% of βlactamaseproducing Staphylococcus aureus and 78.30% of βlactamaseproducing coagulase negative Staphylococcus were oxacillinresistant strains.ConclusionThe high detection rate and high drug resistant rate of βlactamaseproducing Staphylococcus is serious, it is necessary to intensify the monitor and stop quick increase of βlactamase producing Staphylococcus.