ObjectiveTo explore bacterial contamination of direct drinking water system in a hospital.MethodsFrom January 2011 to February 2012, random sampling of finished water, drinking water on clientserver and inner surface of outlet of drinking water machine in a hospital were taken and performed bacterial culture.ResultsThrough the formulation of management system and standardization of operation, maintenance, cleaning and disinfection process of drinking water system, the qualified rate of finished water, drinking water on clientserver,and bacterial culture of inner surface of outlet increased from 93.33% (28/30)to 100.00%(90/90), 89.80%(88/98) to 98.91%(182/184), and 91.11%(41/45) to 100.00% (150/150)respectively (all P<0.05).ConclusionBacterial contamination existed in finished water and drinking water on clientserver, strengthening the standardized disinfection management of drinking water pipes and the clientservers can effectively improve the qualified rate of direct drinking water.