ObjectiveTo investigate an outbreak of healthcareassociated infection(HAI) caused by multidrugresistant Burkholderia cepacia (B.cepacia)， trace the sources and transmission routes of infection, and provide reference for the prevention and control of HAI.MethodsSpecimens from patients and environment were taken and cultured, outbreak was determined based on HAI diagnostic criteria, regional distribution, and exposure time.ResultsFour B.cepacia isolates were isolated from sputum specimens of infected patients, all were multidrugresistant strains. Four infected patients were all from the same ward of the intensive care unit(ICU), their beds were adjacent, onset time was from 16 January to 22 January,2011. Five B.cepacia isolates were isolated from environment specimens, including patients’ward bedside cabinets, ventilator tubes, dressing trolleys, quilts, hands of health care workers, but B.cepacia was not detected from environment in the other wards. Drugresistant spectrum of B.cepacia  from both patients and environment was basically identical.ConclusionThe outbreak of multidrugresistant B.cepacia HAI is due to the contamination of B.cepacia on ICU medical supplies.