ObjectiveTo realize the condition of healthcareassociated infection(HAI)in a hospital，so as to provide reference for preventing and controlling HAI effectively．MethodsAccording to the nationwide HAI prevalence survey project established by ministry of health，HAI prevalence rates were investigated on September 22, 2009, September 10, 2010, and September 7, 2011,respectively, data of three years were compared．ResultsThe prevalence rate of HAI was 4.93%,2.57%, and 2.46% respectively in three years (χ2=8.790，P=0.011), HAI case rate was 5.15%,2.77%, and 3.07% respectively (χ2=7.846，P=0.020), the highest was in 2009，there was significant difference among 3 years. Lower respiratory tract infection was the most common infection, which accounting for 70.37% in 2010, secondly 65.95% in 2009 and 40.00% in 2011. The main pathogens in HAI in 2009 were Pseudomonas aeruginosa (24.00%) and fungi(24.00%)，in 2010 were Klebsiella pneumonia(42.10%) and Staphylococcus aureus (26.31%), and in 2011 were Escherichia coli (20.00%) and Proteus spp.(20.00%); the usage rate of antimicrobial agents was 54.22%,57.45%,and 39.10% respectively in three years；specimen delivery rate was 53.89%,24.11%,and 34.68% respectively.ConclusionSurvey on prevalence rate of HAI is beneficial to realizing the occurrence of HAI, and plays an important role in reducing the occurrence of HAI through monitoring on main departments and main sites of HAI.