ObjectiveTo study the homology of Staphylococcus aureus (S．aureus) isolated from  a mother and her newborn，and to explore the possibility of transmission of S．aureus infection between mother and newborn．MethodsThree S．aureus isolates  from the umbilical secretion of a newborn with omphalitis and from the breast milk of the newborn’s healthy mother were performed  antimicrobial susceptibility testing，homology of S. aureus was analyzed through antimicrobial susceptibility testing phenotyping and  pulsedfield gel electrophoresis (PFGE) genotyping．ResultsThree S．aureus isolates from the mother and newborn had the same antimicrobial susceptibility pattern and PFGE type．ConclusionS．aureus isolated from the mother and newborn were identical strains，suggesting a possibility of S．aureus transmission between mother and newborn．