ObjectiveTo investigate the distribution and antimicrobial resistance of pathogens from patients in a hospital ,and provide basis for rational use of antimicrobial agents.MethodsData about bacterial strains isolated from inpatients and outpatients in a hospital from January 2008 to December 2010 were collected , distribution and antimicrobial resistance of pathogens were analyzed .ResultsA total of 7 008 bacterial strains were isolated, 3 961(56.52%) isolates were gramnegative bacteria , 1 582 (22.57%) were grampositive bacteria, and 1 465(20.91%) were fungi; the top three pathogens were Candida albicans (1 015 isolates, 15.00%) ,Pseudomonas aeruginosa(906 isolates, 12.93%),Escherichia coli(874 isolates, 12.47%). Methicillinresistant Staphylococcus aureus (MRSA) accounted for 85.07%(678/797) of all Staphylococcus aureus, methicillinresistant coagulasenegative Staphylococcus (MRCNS) accounted for 73.17%(150/205) of all CNS; the isolation rate of extendedspectrum βlactamase (ESBLS)producing Escherichia coli and Klebsiella pneumoniae was 64.19%(561/874) and 46.31%(301/650) respectively. Staphylococcus aureus isolates were sensitive to vancomycin and teicoplanin, the resistant rate to sulfamethoxazole/trimethoprim was 68.42％-74.51%, the resistant rate to the other agents were all <30%; isolates of CNS were sensitive to vancomycin and teicoplanin; Escherichia coli and Klebsiella pneumoniae had highest sensitive rate to imipenem and meropenem （95.52％-100.00％）, the resistant rate of Escherichia coli to cefotaxime, ceftriaxone,cefoperazone , ciprofloxacin, and levofloxacin were all ＜30％. Pseudomonas aeruginosa had relative higher resistant rate to ceftazidime (60.31%-85.83%) and meropenem (59.38%-73.23%).ConclusionAntimicrobial resistance of major pathogens isolated from patients in this hospital were generally high . It should pay more attention to the monitoring of multidrug resistant strains, antimicrobial agents should be used rationally.