ObjectiveTo evaluate the occurrence and intervention effectiveness on multidrugresistant organism (MDRO) infection in general intensive care unit (ICU) patients.MethodsTargeted monitor and proper measures on general ICU were performed, the occurrence of MDRO infection before and after infection was compared.ResultsA total of 1 184 patients were investigated, there were 77 cases of healthcareassociated infection(HAI) and 109 communityassociated infection, 186 (15.71%) isolates of MDROs were isolated, the main bacteria were Acinetobacter baumannii (43.55%), methicillinresistant Staphylococcus aureus(29.57%), and Pseudomonas aeruginosa(23.66%). The major infection site was respiratory tract (79.22%, 61/77). After intervention, MDRO infection in this ICU decreased from 6.92%(38/549) to 4.25%(27/635), the difference was significant（χ2=4.04，P＜0.05）.ConclusionMDROs are important pathogens in HAI in general ICU patients, effective measures can reduce the occurrence of MDRO infection.