ObjectiveTo study the distribution and drugresistance of multidrugresistant bacteria(MDRB) from inpatients with acquired immunodeficiency syndrome (AIDS), so as to provide reference for clinical therapy.MethodsAntimicrobial susceptibility testing result of 426 MDRB isolates from AIDS inpatients during 2009-2011 was analyzed by WHONET 5.4 software.ResultsThe isolated MDRB included 213 isolates of extendedspectrum βlactamaseproducing Enterobacteriaceae bacteria, 41 methicillinresistant Staphylococcus aureus (MRSA), 21 methicillinresistant coagulase negative Staphylococcus (MRCNS), 79 MDRAcinetobacter baumannii (MDRAB), and 72 MDRPseudomonas aeruginosa (MDRPA). Except lower drugresistance rate to a minority of broad spectrum antimicrobial agents, ESBLproducing Enterobacteriaceae bacteriaca had higher resistance rates (3.74%-100%） to other antimicrobial agents.ConclusionThe major MDRB from AIDS inpatients is Enterobacteriaceae bacteria, clinicians should choose antimicrobial agents for empirical treatment, surveillance should be intensified, so as to avoid the outbreak of MDR infection in hospital.