ObjectiveTo analyze vancomycin minimum inhibitory concentration (MIC) in methicillinresistant Staphylococcus aureus (MRSA) isolated from different clinical specimens.MethodsFrom 2010 to 2012, MRSA were identified and vancomycin MIC in MRSA was determined by VITEK 2 COMPACT; vancomycin MIC values were analyzed.ResultsA total of 1 434 isolates of Staphylococcus aureus were detected, including 857 sputum specimens, 239 blood specimens,and 338 other specimens; MRSA isolation rate of all specimens was 41.12% (169/411), 44.96% (214/476) and 48.08% (263/547) respectively. MRSA isolation rate of blood specimens was 29.03%,30.86% and 30.21% respectively, and sputum was 46.89%,51.03% and 57.10% respectively; MRSA isolation rate of sputum was higher than that of blood specimens (χ2= 6.41, 10.36, 21.43 respectively, all P<0.05). All strains were sensitive to vancomycin. The percentage of vancomycin MIC≥1.00 μg/mL in MRSA from blood specimens was 38.89%（7 isolates）,40.00%（10 isolates）and 37.93%（11 isolates）in 2010-2012 respectively(χ2=0.02，P=0.999）,and from sputum specimens was 36.28%（41 isolates）, 40.94%（61 isolates） and 49.73% （92 isolates）respectively（χ2=5.72，P=0.057）.ConclusionThe detection rate of MRSA clinical isolates has shown an increased trend from 2010 to 2012. Detection rate of MRSA from sputum was higher than that of blood specimens; detection rate and vancomycin MIC value of MRSA from sputum specimens increased a little; detection rate and vancomycin MIC value of blood specimens didn’t change obviously.