ObjectiveTo evaluate the best method of disinfection and storage time through comparing two different disinfection methods for oxygen humidifier bottles.MethodsFrom March to August 2012, 250 washed oxygen humidifier bottles were randomly divided into control group and experimental group,each group included 125 pieces. Control group was disinfected through immersing into “84” disinfectant containing 500 mg/L of available chlorine; experimental group was disinfected with acidic electrolyzed oxidizing water, bacterial culture results at day 1, day 7-30 and effective storage time of two groups were compared.ResultsAfter being washed, disinfected and dried, all oxygen humidifier bottles of two groups were clear and without water stain. Experimental group had no chlorine smell, but control group had chlorine smell after being immersed.Qualified rates of bacterial monitor of specimens taken at day 1 and 7-12 were all 100.00%; at day 13-30, qualified rate of bacterial monitor was 31.11%(28/90) and 43.33%（39/90） in control group and experimental group respectively（χ2=2.88，P=0.090）.ConclusionTwo disinfection methods can both achieve disinfection efficacy and be effectively stored for 12 days, acidic electrolyzed oxidizing water is perfect, fast acting, safe, nontoxic,and lowcost.