ObjectiveTo investigate the distribution and drug resistance of pathogens isolated from a general surgery department.MethodsThe category,distribution, and drug resistance of pathogens isolated from patients in a general surgery department from January 2011 to December 2012 were analyzed retrospectively.ResultsA total of 859 isolates of pathogens were isolated，563 (65.54%) of which were gramnegative bacteria,233 (27.12%) were grampositive bacteria, and 63 (7.33%)were fungi. The most common gramnegative bacteria was Escherichia coli (40.40%)， the dominant grampositive bacteria was Enterococcus faecalis (3.96%), Candida albicans was the major fungus.Most pathogens were isolated from peritoneal fluid（65.43%，562 isolates）,followed by wound or incisional secretion（10.94%，94 isolates）,sputum （9.90%，85 isolates）, and bile（6.75%，58 isolates）. Enterobacteriaceae were not resistant to imipenem and meropenem,and resistant rates to amikacin, amoxicillin/clavulanic acid,piperacillin/tazobactam and cefoperazone/sulbactam were all <20%,the resistant rates to cephalosporins were high. The resistant rate of Acinetobacter baumannii to imipenem and meropenem was 51.85% and 47.37% respectively，and to most antimicrobial agents were >60%, but to levofloxacin and amikacin were both <20%. The resistant rate of grampositive bacteria to vancomycin was <3.40%.ConclusionThe major pathogens isolated from inpatients in this general surgery department are gramnegative bacteria, survey on distribution and drug resistance of pathogens is important for the rational choice of antimicrobial agents, improvement of therapeutic efficacy,and prevention of the spread of drugresistant bacteria.