Abstract:ObjectiveTo investigate the distribution and antimicrobial resistance of pathogens isolated from blood culture from a hospital.MethodsData of pathogens isolated from blood specimens between 2008 and 2011 were analyzed retrospectively.ResultsA total of 285 pathogenic isolates were obtained from 1 780 specimens, positive rate was 16.01%. The number of grampositive cocci were 155 isolates(54.39%),the major were Staphylococcus aureus (84 isolates,29.48%)and coagulase negative Staphylococcus (38 isolates,12.28%); the number of gramnegative bacilli were 103 isolates(36.14%),the major were Escherichia coli(43 isolates,15.09%),Pseudomonas aeruginosa(19 isolates,6.67%),and Klebsiella pneumoniae(13 isolates,4.56%);the number of fungi was 27 isolates (9.47%),the major was Candida albicans(13 isolates,4.56%).The resistant rate of grampositive cocci was high, but all strains were sensitive to vancomycin and teicoplanin, 40.48% of Staphylococcus aureus and 57.14% of coagulase negative Staphylococcus were methicillinresistant. Gramnegative bacilli were sensitive to imipenem, meropenem,and enzyme inhibitorcontaining antimicrobial drugs; 31 extendedspectrum βlactamaseproducing isolates were detected, 25(58.14%) of which were Escherichia coli, 6(46.15%)were Klebsiella pneumoniae.ConclusionThe major pathogens isolated from blood culture from this hospital are grampositive bacteria, and antimicrobial resistance is high, clinicians should choose antimicrobial agents according to antimicrobial susceptibility testing results, so as to reduce the emergence of resistant strains.