ObjectiveTo investigate antimicrobial resistance of commonly isolated pathogenic bacteria in a hospital between January and December 2012, and compared with report of Ministry of Health National Antimicrobial Resistance Investigation Net(Mohnarin),so as to provide reference for the rational use of antimicrobial agents．MethodsClinical specimens were cultured, minimal inhibitory concentration(MIC) of pathogenic bacteria were detected and compared with the absolute difference in drug resistance rate of Mohnarin report,the overall difference in antimicrobial resistance rates was compared respectively by χ2 test.ResultsIn 2012, the total resistance of four kinds of gramnegative bacteria (Acinetobacter baumannii，Escherichia coli，Klebsiella pneumoniae, Pseudomonas aeruginosa) were slightly higher than Mohnarin report; resistance rate of Staphylococcus aureus was lower than average level of Mohnarin report (P<0.01),detection rate of methicillinresistant Staphylococcus aureus was 19.25%,which was lower than 46.1% of average level of Mohnarin report, vancomycinresistant bacterial strain was not found. Extendedspectrum βlactamaseproducing Escherichia coli and Klebsiella pneumoniae was higher than average level of Mohnarin report respectively(37.54% vs 26.2%; 21.33% vs 16.2%,respectively); multidrugresistant Acinetobacter baumannii and Pseudomonas aeruginosa was 9.2% and 1.5% respectively, which approximating to the detection rate of average level of Mohnarin report.ConclusionAntimicrobial resistance of pathogenic bacteria is stable in this hospital, monitor on bacterial antimicrobial resistance is significant for the rational clinical use of antimicrobial agents.